Journal of Medical Screening > Volume 15, Number 4 > Pp. 199-203

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Automated imaging of circulating fluorocytes for the diagnosis of erythropoietic protoporphyria: a pilot study for population screening

Kin-Chong LauMPhil , Department of Chemical Pathology, Prince of Wales Hospital, Hong Kong, China

Ching-Wan Lam, Associate Professor  , Department of Chemical Pathology, Prince of Wales Hospital, Hong Kong, China

View larger version (38K): [in this window] [in a new window]   Figure 1 IN Cell Analyzer 1000 instrumentation provides quality images and semi-quantitative measurement of protoporphyrin fluorescence in individual erythrocyte for rapid diagnosis of erythropoietic protoporphyria. Patient 1 and Patient 2 inherited the same ferrochelatase mutations in the same family and have similar distribution patterns of fluorocytes. So, only the results of Patient 1 are shown here. Upper panel: images were acquired for 1000-fold phosphate-buffered saline diluted whole blood samples and were pseudocoloured. Fluorocytes in (C) have spherical appearance while fluorocytes in (E) have typical biconcave shape of red cell. The fluorescence intensity of Patient 1's fluorocytes may be too high to mask their biconcave shapes and prolonged storage of the sample at low temperature may lead to shrinkage of the cells. Lower panel: histograms presented with different levels of fluorescence (x-axis), (B) = 0–200 U, (D) and (F) = 0–3100 U, which displayed the whole population of fluorocytes for each sample

 

View larger version (65K): [in this window] [in a new window]   Figure 2 Liquid-based fluorocytes analysis of Hong Kong Chinese population using 96-well-plate approach. The acquired parameters are described previously. Each image of the image thumbnail was autocontrasted independently and pseudocoloured. Partial results of 4013 stored ethylene diamine tetraacetic acid whole blood samples are shown here. Sample well A1 was Patient 3 whole blood sample used as positive control

 

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